a Record control number 000328725100002

a ISSN 1433-6510

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number WA950

b Item number .C66 2013

a Personal name Koksoy, Sadi.

h Medium Electronic Document

a Title Comparison of the Effects of Desflurane and Bupivacaine on Th1 and Th2 Responses

c Statement of responsibility, etc Sadi Koksoy, Zeliha Salih, Bilge Karslı.

b Name of publisher, distributor, etc Clın Lab Publication,

c Date of publication, distribution, etc 2013.

a Summary, etc Background: Anesthesia and surgical trauma are known to affect various functions of the immune system. Alterations reported in the immune system, such as imbalance of Th1 (IFN-gamma) and Th2 (IL-4, 5, 10) cytokines, may result from a number of factors, including pre-medication, type of anesthetic drug, and modality of anesthesia. In this study, we investigated the effects of spinal and general anesthesia with desflurane and bupivakain, respecttively, on Th1 (IFN-gamma) and Th2 (IL-10) cytokines, absolute lymphocyte and natural regulatory T cell numbers (Treg). Methods: Peripheral Blood Mononuclear cells from 24 patients with Benign Prostate Hyperplasia (BPH), undergoing transuretheral prostatectomy under spinal (n = 12) and general (n = 12) anesthesia were analyzed before and 24 hours after surgery. Intracellular cytokine production in response to mitogen stimulation and absolute numbers of Tregs and lymphocytes were determined by using flow cytometry. Results: In patients who received spinal anesthesia, while the frequency of IFN-gamma (1.68% +/- 0.74 vs. 1.03% +/- 0.74) and IL-10 producing CD4(+) T cells decreased (2.62% +/- 2.24 vs. 1.04% +/- 1.06; p < 0.05), the ratio of Th1/Th2 remained similar (1.14 +/- 0.7 vs. 1.52 +/- 1.02; p > 0.05) after surgery. In contrast, in the general anesthesia group the frequency of CD4(+)IFN-gamma(+) T cells increased (1.3% +/- 0.7 vs. 2.5% +/- 1.2; p < 0.05) and the frequency of CD4(+)IL-10(+) T cells decreased (1.1% +/- 0.68 vs. 0.67% +/- 0.47), resulting in an increased Th1/Th2 ratio (1.61 +/- 1.1 vs. 4.77 +/- 3.95; p < 0.05). Absolute lymphocyte and Treg numbers did not change significantly in both groups following surgery. Conclusions: Our results support the notion that general anesthesia, rather than spinal anesthesia, alters the balance of Th1 and Th2 in favor of Th1 responses. However, whether this has any effect on the susceptibility to post-surgery-related infections remains to be determined.

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

a Topical term or geographic name as entry element Medical Laboratory Technology

a Topical term or geographic name as entry element spinal anesthesia

a Topical term or geographic name as entry element general anesthesia

a Topical term or geographic name as entry element Sevoflurane anesthesıa;

a Topical term or geographic name as entry element Propofol ınfusıon

g Relationship information 2013, Volume: 59, Issue: 11-12, Pages: 1215-1220

t Title Clınıcal Laboratory

x International Standard Serial Number 14336510

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.7754/Clin.Lab.2013.120413